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Osteogenic stimulation induces HLA DRA and FTL expression in human VICs. A RT qPCR analysis showing low basal mRNA expression of HLA DRA, FTL, RUNX2, and <t>ALPL</t> under control conditions and significant increases following osteogenic stimulation. B – C Western blot quantification demonstrating parallel increases in protein levels. p < 0.001
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Osteogenic stimulation induces HLA DRA and FTL expression in human VICs. A RT qPCR analysis showing low basal mRNA expression of HLA DRA, FTL, RUNX2, and ALPL under control conditions and significant increases following osteogenic stimulation. B – C Western blot quantification demonstrating parallel increases in protein levels. p < 0.001

Journal: Journal of Computer-Aided Molecular Design

Article Title: Single-nucleus and machine-learning integration identifies HLA-DRA and FTL as immune–metabolic axes and traditional Chinese medicine–targetable hubs in calcific aortic valve disease

doi: 10.1007/s10822-026-00773-x

Figure Lengend Snippet: Osteogenic stimulation induces HLA DRA and FTL expression in human VICs. A RT qPCR analysis showing low basal mRNA expression of HLA DRA, FTL, RUNX2, and ALPL under control conditions and significant increases following osteogenic stimulation. B – C Western blot quantification demonstrating parallel increases in protein levels. p < 0.001

Article Snippet: Osteogenic markers RUNX2 and ALPL were measured using TaqMan Assay IDs Hs01047973_m1 and Hs01029144_m1, respectively.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot